Akademik Veri Yönetim Sistemi
Mediterranean Agricultural Sciences, cilt.30, sa.1, ss.27-33, 2017 (Hakemli Dergi)
Chickpea (Cicer arietinum L.) is an important food legume crop and Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is one of the most important diseases of chickpea in Turkey. Fusarium redolens is known to cause wilt-like disease of chickpea in other countries, but has not been reported from Turkey. Accurate identification of pathogen species and races is important for managing the disease in developing and deploying resistant cultivars. Forty five Fusarium spp. isolates caused wilt of chickpea were obtained from 10 provinces in Turkey from 2007 to 2009. Species level identification of these isolates was done using PCR primers specific for F. oxysporum f. sp. ciceris and F. redolens along with sequencing of the Translation Elongation Factor 1-α (Ef-1α) gene region. Among the isolate collection, eight isolates were identified as F. redolens, and eleven isolates as F. oxysporum f. sp. ciceris, based on PCR with species-specific primers. Seven of the eleven F. oxysporum f. sp. ciceris isolates were further identified as race 0, based on PCR with race-specific primers. Moreover, sequence data of Ef-1α region were used to identify any isolate that did not give amplification with F. oxysporum f. sp. ciceris race specific primers and F. redolens specific primers. This is the first report of F. redolens causing chickpea wilt in Turkey.