Akademik Veri Yönetim Sistemi
World News of Natural Sciences, cilt.54, sa.2024, ss.58-69, 2024 (Hakemli Dergi)
In this study, twenty-eight endophytic fungi were isolated from the roots of the good health Hypericum perforatum plants collected from Samsun, Turkey. Molecular characterization based on sequence variations in the rDNA-ITS (ITS1-5.8S-ITS2) region of 19 from 28 endophytes obtained from H. perforatum was used in genus-level identification. rDNA-ITS sequences were analyzed using the BLAST similarity application on the website of the NCBI. Maximum likelihood rDNA-ITS trees were construct with MEGA11 using the data file of each genus derived from alignments. 13 of the endophytic fungi were determined as Fusarium spp., 5 as Diaporthe spp. and 1 as Dactylonectria spp. To our knowledge, this study was the first report to determine the endophytic fungi in H. perforatum plant from Turkey. Diaporthe spp. and Dactylonectria spp. were determined for the first time in H. perforatum in worldwide with this study. Furthermore, Fusarium spp. was isolated for the first time from H. perforatum in Turkey.
Keywords: Hypericum perforatum, Endophytic fungi, rDNA-ITS region, Phylogeny