Homoarginine, beta-ODAP, and asparagine contents of grass pea landraces cultivated in Turkey

Onar A. N., Yavuz Erdoğan B., Ayan İ., Acar Z.

FOOD CHEMISTRY, cilt.143, ss.277-281, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 143
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1016/j.foodchem.2013.07.051
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.277-281
  • Anahtar Kelimeler: Lathyrus sativus L, beta-ODAP Homoarginine, Asparagine, Capillary electrophoresis, PERFORMANCE LIQUID-CHROMATOGRAPHY, NONPROTEIN AMINO-ACIDS, LATHYRUS-SATIVUS, BETA-N-OXALYL-L-ALPHA,BETA-DIAMINOPROPIONIC ACID, DIAMINOPROPIONIC ACID, THERMAL-ISOMERIZATION, OXALYL DERIVATIVES, NEUROTOXIN, ALPHA, ISOMER
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The seeds of grass pea (Lathyrus sativus L.), a drought tolerant crop, were analysed for quantitative determination of the free amino acids p-N-oxalyl-t-e,3-diaminopropionic acid (beta-ODAP), homoarginine and asparagine by a simple and fast capillary electrophoretic method. In boric acid (80 mM, pH 8.0) running buffer system, not only were a and p-ODAP successfully separated, but also an efficient sample stacking was achieved during hydrodynamic sample introduction. The validated method was used for quantification of p-ODAP, homoarginine and asparagine in seed extracts of 52 Lathyrus local landraces from various regions of Turkey and one released cultivar. The concentration ranges of amino acids were found as 0.211.27% (w/w) for homoarginine, 0.10-0.87% (w/w) for beta-0DAP and 0.006-0.47% (w/w) for asparagine. A positive correlation between homoarginine and 13-0DAP quantities in seeds of 53 Lathyrus local landraces was shown to exist (r2 = 0.649). (C) 2013 Elsevier Ltd. All rights reserved.