Assesment of metal inhibition of antioxidant enzyme glutathione reductase from rainbow trout liver

EKİNCİ D., ŞENTÜRK M.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, cilt.28, sa.1, ss.11-15, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/14756366.2011.615745
  • Dergi Adı: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.11-15
  • Anahtar Kelimeler: Glutathione reductase, rainbow trout, inhibitor, metal ion, OXIDATIVE STRESS, PURIFICATION, DRUGS, IONS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Effects of Co+2, Zn+2, Ca+2, Fe+2, Mn+2, Cr+3, Sn+2 and Mg+2 exposure of rainbow trout liver on the enzymatic activity of glutathione reductase (GR) were investigated. GR was purified from rainbow trout liver 1419-fold with a yield of 38.41% and a specific activity of 22.846 U/mg protein. Following enzyme isolation, inhibitory effects of Co+2, Zn+2, Ca+2, Fe+2, Mn+2, Cr+3 and Sn+2 were analysed. IC50 values of the metal ions ranged from 42.2 to 657 mu M, and the K-i constants ranged from 24.2 to 567 mu. Co+2, Zn+2, Fe+2 and Cr+3 exhibited competitive inhibition whereas others inhibited the enzyme in non-competitive manner. Cobalt was the most powerful inhibitor among others having the lowest IC50 and K-i values. Magnesium exhibited activatory effect on the enzyme.