Microscopic, serologic and molecular surveys on Dirofilaria immitis in stray dogs, Turkey


Simsek S., ÖZKANLAR Y., BALKAYA İ., AKTAŞ M. S.

Veterinary Parasitology, cilt.183, sa.1-2, ss.109-113, 2011 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 183 Sayı: 1-2
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.vetpar.2011.06.012
  • Dergi Adı: Veterinary Parasitology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.109-113
  • Anahtar Kelimeler: Dirofilaria immitis, ELISA, Microscopy, PCR, Prevalance, Turkey
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The prevalance of Dirofilaria immitis infection was evaluated in stray dogs of Erzurum, Turkey. A total of 123 whole-blood and 93 sera samples were collected from stray dogs older than 6 months were lived in animal shelter. The PCR and direct microscopic examinations were used for the detection of microfilaria and indirect-ELISA was performed for the detection of anti- D. immitis antibodies. The prevalence of D. immitis in the canine population was 8.1% by PCR, 2.1% by ELISA. In addition, microfilaria burdens of Dirofilaria sp. was 4.8% by direct blood smear examination. There was a statistical difference (P= 0.05) in the prevalence between males (10.5%) and females (2.3%) by direct blood smear examination. Similarly there was a statistical difference (P< 0.05) in the prevalence between males (15.8%) and females (4.7%) by PCR. Dogs belonging to the 0.5-1 years old group showed the highest prevalence than 2-4 ages group with three tests. Among the 93 samples screened by the ELISA, two samples were positive for the D. immitis antibodies. Both positive dogs with ELISA were females. © 2011 Elsevier B.V.