Development of new total RNA isolation method for tissues with rich phenolic compounds

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Secgin Z., Gokdemir G., Atabay E. S., Kurt Kızıldoğan A., Kavas M.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.45, sa.4, ss.343-350, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 4
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1515/tjb-2019-0375
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.343-350
  • Anahtar Kelimeler: CTAB, hazelnut, phenolics, RNA isolation, RNA sequencing, HIGH-QUALITY RNA, PLANT-TISSUES, WOODY-PLANTS, EXTRACTION, POLYSACCHARIDES
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Background: RNAs to be used in transcriptome analysis must be of high quality and pure in order to ensure maximum representation of the expressed genes. RNA isolation is difficult in hazelnut tissues containing large amounts of secondary metabolite, phenolic compounds and the cell wall structure. Commonly used protocols for RNA isolation are those that require a lot of labor and time and also do not allow sufficient RNA isolation when applied to tissues rich in phenolic compounds. This study was aimed to develop an efficient method for isolation of total RNAs from bud of hazelnut to be used in RNA sequencing.