Effects of Lactic Acid and Chitosan on the Survival of V. parahaemolyticus in Mussel Samples


Terzi Gülel G., Gücükoğlu A.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, cilt.9, sa.6, ss.990-994, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 6
  • Basım Tarihi: 2010
  • Doi Numarası: 10.3923/javaa.2010.990.994
  • Dergi Adı: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.990-994
  • Anahtar Kelimeler: Decontamination, chitosan, lactic acid, mussel, halpphilic marine bacterium, VIBRIO-PARAHAEMOLYTICUS, LISTERIA-MONOCYTOGENES, ANTIBACTERIAL ACTIVITY, ESCHERICHIA-COLI, SHELLFISH, SHRIMP, TDH, TRH, CONTAMINATION, INHIBITION
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Lactic acid and chitosan were evaluated for their effects on the growth and survival of V. parahaemolyticus on mussel meat Mussel (Mytilus sp.) samples were collected from the Samsun region on the middle Black Sea coast of Turkey. Each shelled mussel was decontaminated by immersion in 4% formalin for 3 min. The decontaminated mussel was dipped in TSB broth containing between 8.25 and 7.60 log CFU g(-1) of V. parahaemolyticus and left for 30 min at room temperature 25 degrees C to allow attachment. Initial counts of V. parahaemolyticus in mussel meat immediately after dipping in TSB broth were in the range of 5.38-4.03 log CFU g(-1) Each inoculated mussel (25 degrees C) was dipped in 0.5, 1, 1.5 or 2% of lactic acid (v/v) or 0.05, 0.1, 0.25 or 0.5% of chitosan (v/v) for 5, 15, 30 or 60 min. Initial counts of V. parahaemolyticus in mussel meat decreased following treatment with lactic acid for 5 min by 1.90, 2.13, 2.27 and 2.78 log CFU g(-1), respectively and following treatment with chitosan for 5 min by 1.33, 1.41, 1.56 and >2.03 log CFU g(-1), respectively. Growth of V. parahaemolyticus on mussels was completely inhibited after being dipped in 1.5-2% lactic acid for 15 min and 0.5% chitosan for 5 min.