A brief update on physical and optical disector applications and sectioning-staining methods in neuroscience

Yurt K. K., Kivrak E. G., Altun G., Mohamed H., Ali F., Gasmalla H. E., ...Daha Fazla

JOURNAL OF CHEMICAL NEUROANATOMY, cilt.93, ss.16-29, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 93
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1016/j.jchemneu.2018.02.009
  • Dergi Adı: JOURNAL OF CHEMICAL NEUROANATOMY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.16-29
  • Anahtar Kelimeler: Counting, Neuroscience, Optical and physical disector, Stereology, UNBIASED STEREOLOGICAL ESTIMATION, DESIGN-BASED STEREOLOGY, MEDIAL PREFRONTAL CORTEX, TOTAL NUMBER, LOST CAPS, Z-AXIS, PATHOLOGICAL RESEARCH, ARBITRARY PARTICLES, TISSUE-PREPARATION, NEURONAL NUMBER
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

A quantitative description of a three-dimensional (3D) object based on two-dimensional images can be made using stereological methods These methods involve unbiased approaches and provide reliable results with quantitative data. The quantitative morphology of the nervous system has been thoroughly researched in this context. In particular, various novel methods such as design-based stereological approaches have been applied in neuoromorphological studies. The main foundations of these methods are systematic random sampling and a 3D approach to structures such as tissues and organs. One key point in these methods is that selected samples should represent the entire structure. Quantification of neurons, i.e. particles, is important for revealing degrees of neurodegeneration and regeneration in an organ or system. One of the most crucial morphometric parameters in biological studies is thus the "number". The disector counting method introduced by Steno in 1984 is an efficient and reliable solution for particle number estimation.