Comparison of the proliferative activity in gingival epithelium after surgical treatments of intrabony defects with bioactive glass and bioabsorbable membrane

Cetinkaya B. O., Keles G. C., Ayas B., Aydin O., Kirtiloglu T., Acikgoz G.

CLINICAL ORAL INVESTIGATIONS, cilt.11, sa.1, ss.61-68, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1007/s00784-006-0087-7
  • Dergi Adı: CLINICAL ORAL INVESTIGATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.61-68
  • Anahtar Kelimeler: guided tissue regeneration, bioactive glass, bioabsorbable membrane, long junctional epithelium, proliferating cell nuclear antigen, CELL NUCLEAR ANTIGEN, GUIDED TISSUE REGENERATION, PERIODONTAL DEFECTS, JUNCTIONAL EPITHELIUM, BARRIER MEMBRANE, EXPRESSION, BONE, COMBINATION, OVERGROWTH, ATTACHMENT
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Guided tissue regeneration is based on preventing the more rapidly proliferating epithelium from growing into the periodontal defect after surgical procedures incorporating barrier membranes. The aim of this study was to compare the proliferative activity of gingival epithelium using proliferating cell nuclear antigen (PCNA) as a marker of cell proliferation after surgical treatments with bioactive glass graft material and bioabsorbable membrane. Using split mouth design, 20 intrabony defects were randomly assigned treatments with bioactive glass (BG group) or bioabsorbable membrane (BM group). Gingival biopsies were taken at preoperative and postoperative 12 weeks. After histological processing, the number of the inflammatory cells was measured in hematoxylin and eosin-stained sections; PCNA expression was determined in immunohistochemically-stained sections. At postoperative 12 weeks, the number of the inflammatory cells was significantly decreased (p < 0.01), PCNA expression was significantly increased (p < 0.001) in both treatment groups compared to baseline data. There was no significant difference in PCNA expression between baseline values of two groups (p > 0.05), while at postoperative 12 weeks, increase in BG group was significantly greater than that in BM group (p < 0.001). These results suggest that epithelial cell proliferation is more prominent after treatment of intrabony defects with bioactive glass compared to the treatment with bioabsorbable membrane.