Atıf İçin Kopyala
Taşkurt Hekim G. N., Dolu Z., Güneş S., Aşçı R.
Experimental Biomedical Research, cilt.7, sa.1, ss.1-8, 2024 (Hakemli Dergi)
Özet
Aim: Toevaluate the relationship between sperm chromatin condensation assessed by aniline blue staining and semen parameters in infertile men.Methods:Infertile men applied to our urology clinics and diagnosed with normozoospermia (n=50), asthenozoospermia (n=17), oligozoospermia (n=3), teratozoospermia (n=2) oligoasthenoteratozoospermia (OAT) (n=10) according to their semen analysis were included in the study. Semen samples were evaluated for sperm chromatin condensation by aniline blue staining.Results:The percentage of aniline-positive spermatozoa in the OAT and teratozoospermia group was found to be higher than in the normozoospermic and asthenozoospermic infertile groups (p=0.002, p=0.044 with normozoospermia group and p=0.026, p=0.007 with asthenozoospermia group, respectively). Sperm chromatin condensation was negatively correlated with sperm concentration (p=0.003, r=-0.322), total sperm count (p=0.004, r=-0.313), total progressive motile sperm count (p=0.005, r=-0.307), and normal morphology (p<0.0001, r=-0.554); and positively correlated with the percentage of immotile sperm (p=0.037, r=0.230).Conclusion:Sperm chromatin condensation was found to be different in infertile men differently diagnosed based on their semen analysis. The results of the study suggest that chromatin condensation, together with routine sperm parameters, may constitute a valuable parameter in the evaluation of male fertility.Keywords: Aniline blue, oligoasthenoteratozoospermia, semen analysis, sperm chromatin.*Neslihan Hekim, Department of Medical Biology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TürkiyeE-mail: neslihan.taskurt@omu.edu.trReceived: 2023-09-14Accepted: 2023-11-20/ Published: 2024-01-01Infertility is a health problem in which couples cannot achieve pregnancy despite at least one year of unprotected and regular sexual intercourse [1]. According to global infertility prevalence estimates by World Health Organization (WHO) for 2022, infertility affectsalmost one in six people worldwide at some point in their life [2]. Approximately in the half of the couples with struggling infertility, the problems are caused by male partners [3]. Routine semen analysis, together with the medical history and physical examination, are essential elements in the evaluation of male infertility. Semen analysis provides information aboutsperm count, sperm motility and viability, sperm morphology, and functional status of accessory sex glands. However, semen analysis may be limited in evaluating male fertility chances [4].The reorganization of sperm chromatin is a sperm-specific epigenetic rearrangement and Experimental Biomedical ResearchOriginal articleIntroduction Aim: Toevaluate the relationship between sperm chromatin condensation assessed by aniline blue staining and semen parameters in infertile men.Methods:Infertile men applied to our urology clinics and diagnosed with normozoospermia (n=50), asthenozoospermia (n=17), oligozoospermia (n=3), teratozoospermia (n=2) oligoasthenoteratozoospermia (OAT) (n=10) according to their semen analysis were included in the study. Semen samples were evaluated for sperm chromatin condensation by aniline blue staining.Results:The percentage of aniline-positive spermatozoa in the OAT and teratozoospermia group was found to be higher than in the normozoospermic and asthenozoospermic infertile groups (p=0.002, p=0.044 with normozoospermia group and p=0.026, p=0.007 with asthenozoospermia group, respectively). Sperm chromatin condensation was negatively correlated with sperm concentration (p=0.003, r=-0.322), total sperm count (p=0.004, r=-0.313), total progressive motile sperm count (p=0.005, r=-0.307), and normal morphology (p<0.0001, r=-0.554); and positively correlated with the percentage of immotile sperm (p=0.037, r=0.230).Conclusion:Sperm chromatin condensation was found to be different in infertile men differently diagnosed based on their semen analysis. The results of the study suggest that chromatin condensation, together with routine sperm parameters, may constitute a valuable parameter in the evaluation of male fertility.Keywords: Aniline blue, oligoasthenoteratozoospermia, semen analysis, sperm chromatin.*Neslihan Hekim, Department of Medical Biology, Faculty of Medicine, Ondokuz Mayis University, Samsun, TürkiyeE-mail: neslihan.taskurt@omu.edu.trReceived: 2023-09-14Accepted: 2023-11-20/ Published: 2024-01-01Infertility is a health problem in which couples cannot achieve pregnancy despite at least one year of unprotected and regular sexual intercourse [1]. According to global infertility prevalence estimates by World Health Organization (WHO) for 2022, infertility affectsalmost one in six people worldwide at some point in their life [2]. Approximately in the half of the couples with struggling infertility, the problems are caused by male partners [3]. Routine semen analysis, together with the medical history and physical examination, are essential elements in the evaluation of male infertility. Semen analysis provides information aboutsperm count, sperm motility and viability, sperm morphology, and functional status of accessory sex glands. However, semen analysis may be limited in evaluating male fertility chances [4].The reorganization of sperm chromatin is a sperm-specific epigenetic rearrangement and Experimental Biomedical ResearchOriginal articleIntroduction