Akademik Veri Yönetim Sistemi
International Journal of Chemistry and Technology (IJCT), cilt.6, sa.2, ss.164-169, 2022 (Hakemli Dergi)
Hypericum perforatum, known as St. John's Wort and most used ethnaformacolgically, belongs to the Hypericaceae family. In this work, we aimed to evaluate the antioxidant, antimicrobial, DNA protective activities, and enzyme inhibitor properties of domestic and import H. perforatum aqueous extracts. The total phenolic and flavonoid contents were also determined for both samples. Results exhibited a high phenolic content for Domestic H. perforatum aqueous extract (DHPE) and Import H. perforatum aqueous extract (IHPE) samples. Meanwhile, inhibition activity levels for α-glucosidase were remarkable, the IC50 value of DHPE was 16.35±0.07 µg/mL, and the IC50 value of IHPE was 15.05±1.36 µg/mL, both samples demonstrated almost twice of the standard inhibitor effect (IC50: 30.62±2.07 µg/mL). Moreover, the inhibition activity of both samples against α-amylase was more effective than acarbose. The antibacterial results were as follows; the highest MIC value detected by IHPE was 64 µg/mL against B. cereus. In addition, IHPE was shown to be more effective than the domestic sample against all microorganisms. Furthermore, DHPE exhibited good protective activity from oxidative H2O2-induced DNA damage. Both tested samples had an abundance of phenolic content and were high in inhibitory activity against diabetic enzymes. Generally, bioactivity tests’ results appeared quite effective for both samples compared to the standards.