CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

Cardi T., Murovec J., Bakhsh A., Boniecka J., Bruegmann T., Bull S. E., ...Daha Fazla

Trends in Plant Science, cilt.28, sa.10, ss.1144-1165, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Derleme
  • Cilt numarası: 28 Sayı: 10
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.tplants.2023.05.012
  • Dergi Adı: Trends in Plant Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Environment Index, MEDLINE, Veterinary Science Database, DIALNET
  • Sayfa Sayıları: ss.1144-1165
  • Anahtar Kelimeler: CA18111, CRISPR applications, CRISPR platforms, gene regulation, mutant detection, plant regeneration, PlantEd
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.