Akademik Veri Yönetim Sistemi
Kocatepe Veterinary Journal, cilt.11, sa.4, ss.430-433, 2018 (Hakemli Dergi)
Canine demodicosis (CD) is an inflammatory skin disease caused by excessive proliferation of Demodex mites (Acarina:Demodicidae) former in the dogs’ follicles latter in the sebaceous glands. To date Demodex canis, D. injai, and D. cornei arethe three Demodex species that recognized in the domestic dog Canis familiaris. There are reports of morphologicalidentification of Demodex spp. from pet dogs in Turkey. Nevertheless there had been no reports of characterizing theDemodex mite from pet dogs in Turkey using well-defined mitochondrial DNA gene sequences. The aim of the presentstudy is to reveal first molecular characterization data on D. canis infesting domestic dogs (Canis familiaris) in Turkey.Demodex mites were obtained from skin scrapings of dogs with CD and identified microscopically as D. canis. Themitochondrial DNA 12S (mtDNA 12S) rRNA gene amplified, sequenced and compared with available Demodex sequencesin Genbank using BLAST analyses. D. canis isolate (DP-Samsun, MH374631) obtained in the study matched 100%(474/474) with previously reported gene sequences for the 12S rRNA of mtDNA in D. canis (KX264486, isolateDemo.can2) from dogs in China. Pairwise comparison between the 12S rRNA sequences of the D. canis from Turkey(MH374631) and other mtDNA rRNA gene of D. canis isolates from China (KX264486-88) presented differences rangingfrom 0.0 to 0.6 %. In conclusion, within the present study, we provided the molecular characterization of D. canis indomestic dogs for the first time by sequencing of the partial mtDNA in Turkey.