A recombinant PvpA protein-based diagnostic prototype for rapid screening of chicken Mycoplasma gallisepticum infections

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Bueyuektanir Ö., YILDIRIM T., Yakicier C., Genç O., Yurdusev N.

VETERINARY MICROBIOLOGY, cilt.129, sa.1-2, ss.139-149, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 129 Sayı: 1-2
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.vetmic.2007.11.028
  • Dergi Adı: VETERINARY MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.139-149
  • Anahtar Kelimeler: Mycoplasma gallisepticum, recombinant PvpA, serodiagnostic prototype, chicken, MONOCLONAL-ANTIBODY, ANTIGEN, CYTADHESIN, EXPRESSION, ASSAY, GENE, HEMAGGLUTININS, STRAINS, EPITOPE, CLONING
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Mycoplasma gallisepticum is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Serological monitoring is essential to maintain mycoplasma-free breeder flocks and often complicated by the cross-reactions between different mycoplasma species. To overcome serological cross-reactions, a large fragment of the M. gallisepticum PvpA cytadhesin, species-specific surface-exposed protein, was produced in E. coli as a recombinant protein (rPvpA336) and used as a potential diagnostic antigen. The rPvpA336 protein possesses 336 mycoptasma-specific amino acids with relative molecular weight of 44 kDa. A deletion region of 37 amino acids was identified when compared to the wild-type PvpA protein. Immunoreactivity of the rPvpA336 protein has been demonstrated by Western blot analysis with M. gallisepticum-positive and -negative chicken sera. Furthermore, an enzymatic rapid immunofiltration assay (ERIFA) prototype based on the rPvpA336 protein has been developed and its species-specific detection capability has been demonstrated by using M. gallisepticum and/or M. synoviae-positive and -negative chicken sera. In addition to its species-specificity, the ERIFA prototype presents certain advantages such as rapidity, field-applicability and cost-effectiveness. Therefore, these advantages would make the prototype a species-specific rapid diagnostic tool of choice in the field and limited laboratory conditions for screening M. gallisepticum infections. (C) 2007 Elsevier B.V. All rights reserved.