Morphological and molecular characterization of <i>Hysterothylacium</i> larval morphotypes (Nematoda: Raphidascarididae) infecting edible marine fish in the Black Sea

Gelen M. Y., Pekmezci G. Z.

PARASITOLOGY RESEARCH, cilt.122, sa.8, ss.1863-1872, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 122 Sayı: 8
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s00436-023-07887-3
  • Dergi Adı: PARASITOLOGY RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1863-1872
  • Anahtar Kelimeler: Hysterothylacium larval morphotypes, ITS regions, cox2 gene, Sequencing, Black Sea, MERLANGIUS-MERLANGUS, ADUNCUM NEMATODA, COASTS, ASCARIDOIDEA, CONFIDENCE, ANISAKIDAE, ALIGNMENT
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The morphological and molecular identification of Hysterothylacium larval morphotypes in the Black Sea remains unresolved and incomplete. The aim of current study was to provide a detailed morphological identification with rDNA whole ITS (ITS-1, 5.8S subunit, ITS-2) and mtDNA cox2 sequences data of Hysterothylacium larval morphotypes infecting four commonly edible marine fish species, including European anchovy, Engraulis encrasicolus (L.), horse mackerel, Trachurus trachurus (L.), whiting, Merlangius merlangus (L.), and red mullet, Mullus barbatus ponticus (E.) in the Black Sea (FAO fishing area 37.4.2). Hysterothylacium larval morphotypes were morphologically classified, followed by whole ITS and cox2 sequencing. Four Hysterothylacium larval morphotypes, III, IV, VIII, and IX, are described based on morphological and molecular data. The present study provides the first study reporting whole ITS and cox2 sequences for Hysterothylacium larval morphotypes III, IV, VIII and III, IV, VIII, IX, respectively, in the Black Sea. Here, we offer a foundation for future research on the distribution, morphologic and molecular identification of Hysterothylacium larval morphotypes infecting edible some marine fish in the Black Sea.