Homologous expression of lysA encoding diaminopimelic acid (DAP) decarboxylase reveals increased antibiotic production in Streptomyces clavuligerus

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Otur Ç., Kurt Kızıldoğan A.

BRAZILIAN JOURNAL OF MICROBIOLOGY, cilt.51, sa.2, ss.547-556, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 51 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1007/s42770-019-00202-2
  • Dergi Adı: BRAZILIAN JOURNAL OF MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.547-556
  • Anahtar Kelimeler: Streptomyces clavuligerus, lysA, Overexpression, Cephamycin C, Clavulanic acid, Tunicamycin, LYSINE EPSILON-AMINOTRANSFERASE, CEPHAMYCIN-C, CLAVULANIC ACID, HETEROLOGOUS EXPRESSION, ESCHERICHIA-COLI, BIOSYNTHESIS, TUNICAMYCIN, GENES, CCAR, CLUSTER
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

lysA gene encoding meso-diaminopimelic acid (DAP) decarboxylase enzyme that catalyzes l-lysine biosynthesis in the aspartate pathway in Streptomyces clavuligerus was overexpressed, and its effects on cephamycin C (CephC), clavulanic acid (CA), and tunicamycin productions were investigated. Multicopy expression of lysA gene under the control of glpF promoter (glpFp) in S. clavuligerus pCOlysA led to higher expression levels ranging from 2- to 6-fold increase at both lysA gene and CephC biosynthetic gene cluster at T-36 and T-48 of TSBG fermentation. These results accorded well with CephC production. Thus, 1.86- and 3.14-fold higher volumetric as well as 1.26- and 1.71-fold increased specific CephC yields were recorded in S. clavuligerus pCOlysA in comparison with the wild-type and its control strain, respectively, at 48th h. Increasing the expression of lysA provided 4.3 times more tunicamycin yields in the recombinant strain. These findings suggested that lysA overexpression in S. clavuligerus made the strain more productive for CephC and tunicamycin. The results also supported the presence of complex interactions among antibiotic biosynthesis pathways in S. clavuligerus.