Akademik Veri Yönetim Sistemi
Akademik Ziraat Dergisi, cilt.7, sa.1, ss.21-28, 2018 (Hakemli Dergi)
Bacillus megaterium isolates, were demonstrated tobe efficient biocontrol agents against the greenshield bug (Palomena prasina L., Heteroptera:Pentatomidae). Firstly hazelnut orchards weresurveyed and four B. megaterium isolates wereobtained from P. prasina. The morphological,physiological and biochemical characteristics of B.megaterium isolates were determined according tothe standardized methodology. Additionally 16SrRNA gene sequence analyse was performed todetermine the isolates. Analysis of the 16S rRNAgene confirmed that isolates Sa-1, Sa-5, SAk-2 andSAkc-19 are B. megaterium, with 100% sequencehomology to the type strains of B. megaterium.Effectiveness of these isolates was tested against P.prasina nymphs in laboratory, at 25±1°C and 70±5RH. Isolates were bioassayed against nymphs anddead individuals were counted daily, following a 12days lasting treatment. Lethal time (LT50 and LT90)values of B. megaterium isolates were calculated.LT50 values of the two most active isolates were 1.91and 11.18 days. The highest mortality rate was 98%obtained with the treatment of SAkc-2 isolates atconcentrations of 108 cfu ml-1 on the 12th day of posttreatment.