Isolation, characterization and molecular identification of a halotolerant Bacillus megaterium CTBmeg1 able to grow on halogenated compounds

---

Atıf İçin Kopyala

Akcay K., Kaya Y.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, cilt.33, sa.1, ss.945-953, 2019 (SCI-Expanded)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 33 Sayı: 1
• Basım Tarihi: 2019
• Doi Numarası: 10.1080/13102818.2019.1631717
• Dergi Adı: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
• Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
• Sayfa Sayıları: ss.945-953
• Anahtar Kelimeler: Biodegradation, halogenated compounds, 2, 2-dichloropropionic acid, dehalogenase, Bacillus megaterium, hypersaline environments, L-HALOACID DEHALOGENASE, HALOALKANE DEHALOGENASE, MICROBIAL DIVERSITY, HALOPHILIC BACTERIA, SP NOV., LAKE, BIODEGRADATION, ENVIRONMENTS, PURIFICATION, POLLUTANTS
• Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Halogenated compounds pose long-term potential risks to the well-being of humans due to their recalcitrance and persistent toxicity. The quest for microorganisms capable of degrading such perilous substances merits urgent consideration. In this study, a new dehalogenase-producing bacterium was isolated from a hypersaline environment (TuzGolu Lake, Turkey), and identified as Bacillus megaterium strain CTBmeg1 (Accession number MK128900). Under culture conditions (pH 8.0, NaCl 20%, 30 degrees C, 200 rpm, 9 days), the B. megaterium strain CTBmeg1 showed an optimum growth on 10 mmol/L of 2,2-dichloropropionic acid with a doubling time of 26.41 h. Furthermore, the presence of a putative halotolerant dehalogenase gene (dehCTBmeg1) of B. megaterium strain CTBmeg1 was detected and amplified via PCR technique. Bio-prospecting for microorganisms in a highly saline environment capable of utilizing halogenated compounds as the sole carbon may prove to be a practical and safer means for bioremediation of contaminated coastal areas, an increasingly common place predicament faced by many nations.