RAPID IMMUNOFILTRATION ASSAY AS A FIELD DIAGNOSTIC TOOL FOR OVINE BRUCELLOSIS

Büyüktanır Yaş Ö., Genç O., ÇELEBİ Ö., Yurdusev N.

JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY, cilt.33, sa.1, ss.35-47, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 1
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1080/15321819.2011.591478
  • Dergi Adı: JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.35-47
  • Anahtar Kelimeler: CFT, diagnosis, ELISA, ovine brucellosis, rapid immunofiltration assays, RBT, LINKED-IMMUNOSORBENT-ASSAY, MELITENSIS INFECTION, BOVINE BRUCELLOSIS, SEROLOGICAL DIAGNOSIS, IMMUNOBLOT ANALYSIS, ROSE-BENGAL, ABORTUS, SHEEP, PROTEIN, ANTIBODY
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

This work describes the development of two rapid immunofiltration assays, enzymatic (ERIFA) and non-enzymatic (NERIFA), for the rapid detection of ovine anti-Brucella antibodies. Brucella abortus lipopolysaccharide and total bacterial extract were dotted separately as diagnostic antigens on a nitrocellulose filter-membrane of the individual assay unit along with a third dot of purified sheep IgG as an internal control. The assay's diagnostic performance was evaluated in comparison with a modified rose bengal test (mRBT) and an indirect enzyme-linked immunosorbent assay (ELISA) through usage of 590 serum samples from healthy, vaccinated, or infected sheep. The ERIFA and indirect ELISA were found to be significantly more sensitive than NERIFA, while mRBT was determined to be statistically equivalent to NERIFA. A perfect agreement (kappa = 0.984) and a statistical equivalence to indirect ELISA suggest that the bi-antigenic ERIFA can be used as an "individual rapid ELISA'' for screening ovine anti-Brucella antibody both in the field and in limited laboratory conditions.