Prediction models for the phenolic contents in some Hypericum species from Turkey


ODABAŞ M. S., Radusiene J., Cirak C., Camas N.

ASIAN JOURNAL OF CHEMISTRY, cilt.20, sa.6, ss.4792-4802, 2008 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 6
  • Basım Tarihi: 2008
  • Dergi Adı: ASIAN JOURNAL OF CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4792-4802
  • Anahtar Kelimeler: Hypericum originafolium, Hypericum perfoliatum, Hypericum montbreii, modelling, phenolic contents, plant growth stages, ST-JOHNS-WORT, SECONDARY METABOLITES, PERFORATUM
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

In this research, models for prediction of the content of several phenolics namely chlorogenic acid, hyperoside, apigenin-7-O-glucoside, rutin, quercitrin, quercetin and viteksin were developed for Hypericum originafolium Willd, Hypericum perfoliatum L. and Hypericum montbreii Spach. growing in Northern Turkey. Wild growing plants were harvested at vegetative, floral budding, full flowering, fresh fruiting, mature fruiting stages and dissected into stem, leaf and reproductive tissues. Actual phenolic content of plant materials was measured by high performance liquid chromatography method. Multiple regression analysis with Excel 2003 computer package program was performed for each species and phenolic separately to develop the models. The produced equation for predicting of phenolic content in different tissues of the species was formulized as: PC= [a + (b(1) x S) + (b(2) x L) + (b(3) x R) + (b(4) X S-2) + (b(5) x (1/RP))] where PC is whole plant content of phenolic compound, S is phenolic content of stem, L is phenolic content of leaf, RP is phenolic content of reproductive parts and a, b(1,) b(2), b(3), b(4) and b(5) are coefficients. R-2 values varied between 0.65-0.99 for H. originafolium, 0.67-0.99 for H. perfoliatum and 0.96-0.99 for H. montbreii depending of the phenolics examined. All R-2 values and standard errors were found to be significant at the p < 0.05 level.