Akademik Veri Yönetim Sistemi
THERIOGENOLOGY, cilt.86, sa.2, ss.572-578, 2016 (SCI-Expanded)
The purpose of this study was to compare the expression of 3 beta-hydroxystreroid dehydrogenase (3 beta-FISD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the control group. For immunostaining a rabbit polyclonal, one raised against recombinant human type 2 (adrenal/gonadal) 3 beta-HSD was used. Progesterone (P4) concentrations were not statistically different between the groups. Strongly stained large interstitial cell groups in the ovarian medulla were observed particularly in CEH-pyometra group although these cells in the control group were weakly or moderately stained and existed singly or paired. The expressions of 3 beta-HSD in luminal epithelium (42.40 +/- 22.40% vs. 18.42 +/- 13.15%, P < 0.05) and glandular epithelium (32.80 +/- 27.05% vs. 2.94 +/- 7.79%, P < 0.01) of endometrium were significantly higher in CEH-pyometra group than those in the control group. The expression of 3 beta-HSD in CL was higher (29.38 +/- 9.58% vs. 22.94 +/- 4.97%) in CEH-pyometra group than that of control group although the differences were not significant (P > 0.05). Similarly, the significant increase in the expression of 3 beta-HSD in ovarian interstitial cells (33.86 +/- 29.44 vs. 1.13 +/- 2.97, P < 0.05) was found in CEH-pyometra group compared to the control group. The study revealed that 3 beta-HSD expression in the endometrium of canine CEH-pyometra was significantly high. (c) 2016 Elsevier Inc. All rights reserved.