The existence of Listeria monocytogenes in a cattle slaughterhouse and identification of serotypes by mPCR

ÇADIRCI Ö., Gücükoğlu A., Terzi Gülel G., Uyanık T., Alişarlı M.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.65, sa.3, ss.305-311, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 65 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1501/vetfak_0000002861
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.305-311
  • Anahtar Kelimeler: IMS, Listeria monocytogenes, serotyping, slaughterhouse, SALMONELLA SPP., FOOD, EQUIPMENT, MEAT, PLANTS, BEEF, CONTAMINATION, PREVALENCE, CARCASSES, PERSONNEL
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

This study was carried out to investigate the presence of Listeria monocytogenes in samples obtained from a slaughterhouse in Samsun, Turkey. A total of 300 samples (25 samples per month) analyzed by Immunomagnetic Separation (IMS) based on conventional culture method. Then isolates were verified and serotyped by PCR using specific primers. According to the analysis, a total of 18 isolates were identified as L. monocytogenes Distribution of these isolates were as;5 isolates (serotype l/2b or 3b) from 1 cattle carcass, 5 isolates (serotype l/2b or 3b) from 1 knife, 2 isolates (serotype 4b or 4d, 4e) from 1 knife sharpener, 5 isolates (serotype l/2a or 3a) from 1 floor sample, 1 isolate (serotype l/2a or 3a) from 1 cowhide, respectively. As a result, carcasses are considered to pose a hazard to public health due to L. monocytogenes contamination. The development of slaughterhouse conditions and production technology, improving the education level of employees in food safety issues, prevention of contamination of foodborne pathogens to carcasses in slaughterhouses and implementation of effective sanitation methods should be provided.