In vitro antioxidant, DNA-damaged protection and antiproliferative activities of ethyl acetate and n-butanol extracts of Centaurea sphaerocephala L.

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Lahneche A. M., Boucheham R., Özen T., ALTUN M., Boubekri N., Demirtaş İ., ...Daha Fazla

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS, cilt.91, sa.3, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 91 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1590/0001-3765201920180462
  • Dergi Adı: ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: Centaurea sphaerocephala, phenolics, antioxidant, lipid peroxidation, antiproliferative, DNA-damaged inhibition, BIOACTIVITY-GUIDED ISOLATION, TOTAL PHENOLIC CONTENT, SESQUITERPENE LACTONES, CHEMICAL-CONSTITUENTS, SECONDARY METABOLITES, FLAVONOID AGLYCONES, PLANT, ANTICANCER, BOISS., COSS.
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

This study aimed to evaluate the in vitro antiproliferative and inhibition of oxidative DNA-damage activities of n-butanol (n-BuOH) extract of Centaurea sphaerocephala. The in vitro antioxidant activity of the ethyl acetate (EtOAc) and the n-BuOH extracts of this plant were also assayed. To investigate the antioxidant potential, extracts were tested for their capacity to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to inhibit lipid peroxidation using the TBARs method. The contents of total phenolics and flavonoids were measured. Additionally, antiproliferative activity and DNA-damage inhibition of the n-BuOH extract was determined using XCELLigence RTCA instrument and photolyzing 46966 plasmid. respectively. The results exhibited that the scavenging abilities of the EtOAc extract were better than the n-BuOH extract with an IC50 = 11.59 mu g/mL and 16.67 mu g/mL for both extracts, respectively. The phenolic and flavonoid contents were found higher in the n-BuOH and EtOAc extracts. Furthermore, our results showed that n-BuOH extract exhibited a remarkable inhibition of lipid peroxidation with an IC50 of 340.94 +/- 7.49 mu g/mL and had an antiproliferative effect against Hela cells. Extracts of C. sphaerocephala showed antioxidant activity on scavenging DPPH. In addition, the n-BuOH extract inhibited the lipid peroxidation and exhibited an antiproliferative effect against HeLa cells line (human cervix carcinoma).