A rapid detection of multidrug-resistant Mycobacterium tuberculosis by a nitrate reductase assay on blood agar

Creative Commons License

Coban A. Y., Tanrıverdi Çaycı Y., Deveci A., Akgunes A., UZUN M., Durupinar B.

MEMORIAS DO INSTITUTO OSWALDO CRUZ, cilt.106, sa.3, ss.378-380, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 106 Sayı: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1590/s0074-02762011000300022
  • Dergi Adı: MEMORIAS DO INSTITUTO OSWALDO CRUZ
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.378-380
  • Anahtar Kelimeler: Mycobacterium tuberculosis, nitrate reductase assay, blood agar, COLORIMETRIC METHOD, LIQUID CULTURES, SPUTUM SAMPLES, SUSCEPTIBILITY, RESAZURIN, RIFAMPICIN, DRUGS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The susceptibility of 49 Mycobacterium tuberculosis clinical isolates to isoniazid (INH) and rifampisin (RIF) (28 multi-drug resistant-tuberculosis samples) was determined by a nitrate reductase assay (NRA) on blood agar. Agreement between the NRA and other testing methods was found to be 93.8% for both INH and RIF. The sensitivity, specificity, positive predictive value and negative predictive value for INH were 92.8%, 94.2%, 86.6% and 97%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value for RIF were 90.4%, 96.4%, 95% and 93.1%. In conclusion, we show here that blood agar can be used effectively for the NRA test.