A comparative study of parthenogenetic activation and in vitro fertilization of in vitro matured bovine oocytes

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Çevik M., Tas A., Akkoc T., Bagis H., Arat S.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.33, sa.5, ss.393-399, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 5
  • Basım Tarihi: 2009
  • Doi Numarası: 10.3906/vet-0803-5
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.393-399
  • Anahtar Kelimeler: Maturation, in vitro fertilization, parthenogenetic oocyte activation, embryo culture, NUCLEAR TRANSFER EMBRYOS, CHEMICAL ACTIVATION, ETHANOL, SPERM, CYCLOHEXIMIDE, PLOIDY, CATTLE, SERUM
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The objective of this study was to determine the effects of 3 chemical agents used sequentially and electrical stimuli on parthenogenetic activation of in vitro matured bovine oocytes and comparison with a standard IVF protocol for embryo developmental rates. For this purpose, oocytes were matured in tissue culture medium-199 (TCM-199) at 39 C and 5% of CO(2) in humidified air. For IVF, matured oocytes were fertilized in Modified Tyrode-Lactate Medium. In the parthenogenetic activation process, direct current (DC) was pulsed (133 V/500 mu m) for 25 mu s and oocytes were sequentially activated with calcium ionophore (CaI) for 10 min, cycloheximide (CHX) + cytochalasin D for 1 h, and CHX for 5 h. After that, all embryos (both IVF and parthenogenetic) were cultured in G1.3/G2.3 media containing 6% CO(2), 5% O(2), and 89% N(2) in humidified air at 39 degrees C.