In-depth comparative transcriptome analysis of Purpureocillium sp. CB1 under cadmium stress

Kurt-Kızıldoğan A., Otur Ç., Yıldırım K., Kavas M., Abanoz-Seçgin B.

Applied Microbiology and Biotechnology, cilt.107, sa.17, ss.5453-5467, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 107 Sayı: 17
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s00253-023-12655-5
  • Dergi Adı: Applied Microbiology and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.5453-5467
  • Anahtar Kelimeler: Bioremediation, Cadmium, Leucinostatin, Purpureocillium sp. CB1, RNA-seq, Salicylate hydroxylase
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Abstract: Fungal bioremediation is a very attractive tool to cope with environmental pollution. We aimed to decipher the cadmium (Cd) response of Purpureocillium sp. CB1, isolated from polluted soil, at transcriptome level by RNA-sequencing (RNA-seq). We used 500 and 2500 mg/L of Cd2+ concentrations at two time points (t6;36). RNA-seq determined 620 genes that were co-expressed in all samples. The highest number of differentially expressed genes (DEGs) was obtained within the first six h of exposure to 2500 mg/L of Cd2+. Several genes encoding transcriptional regulators, transporters, heat shock proteins, and oxidative stress-related genes were differentially expressed under Cd2+ stress. Remarkably, the genes that encode salicylate hydroxylase, which is involved in naphthalene biodegradation pathway, were significantly overexpressed. Utilization of diesel as the sole carbon source by CB1 even in the presence of Cd2+ supported concomitant upregulation of hydrocarbon degradation pathway genes. Furthermore, leucinostatin-related gene expression levels increased under Cd2+ stress. In addition, leucinostatin extracts from Cd2+-treated CB1 cultures showed higher antifungal activity than the control. Notably, Cd2+ in CB1 was mainly found as bound to the cell wall, thus confirming its adsorption potential. Cd2+ stress slightly reduced growth and led to mycelial malformation due to Cd2+ adsorption, especially at a concentration of 2500 mg/L at t36. A strong correlation was recorded between RNA-seq and reverse-transcriptase-quantitative polymerase chain reaction (RT-qPCR) data. In conclusion, the study represents the first transcriptome analysis of Purpureocillium sp. under Cd2+ stress, providing insights into the primary targets for rational engineering to construct strains with remarkable bioremediation potency. Key points: • Upregulation of genes encoding salicylate hydroxylases under Cd 2+ stress • Maximum Cd 2+ adsorption at 500 mg/L at t36 as tightly bound to the cell wall • Concordant bioremediation potential of CB1 on Cd 2+ and diesel Graphical abstract: [Figure not available: see fulltext.].