Akademik Veri Yönetim Sistemi
KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.25, ss.591-596, 2022 (ESCI)
The aim of this study was to determine the effects of shilajit (S) at different doses (0, 5, 10, 15, 20%) added to honey bee (Apis melilifera) semen extender on motility, plasma membrane integrity and acrosome integrity parameters. In the study, a total of 25 colonies, 5 groups and 5 colonies in each group, were used. The control group (S-0) constitutes the group where shilajit was not added to the honeybee extender. S- 1, S- 2, S-3 and S-4 groups were formed as groups with 5, 10, 15 and 20% shilajit added to the honey bee extender, respectively. Sperm were collected from approximately 400 drones aged 14-21 days in approximately 25 colonies that were fed every other day. Then they were frozen in liquid nitrogen vapor and stored in liquid nitrogen at -196 degrees C for approximately 7 months. At the end of this period, the frozen samples were thawed at 37 degrees C and examined in terms of relevant parameters. Compared with other groups, S-3 and S-4 groups increased sperm motility (p<0.05); It was determined that S-2, S-3 and S-4 groups increased plasma membrane integrity while the acrosome integrity was significantly preserved in the groups with different doses of shilajit added to the diluent compared to the control group (p<0.01). As a result, it was concluded that different doses of shilajit to honey bee extender had positive effects on spermatological parameters.