Real-time assessment of the superovulatory effect of FSH and eCG with laparoscopy at different seasons in Akkaraman ewes

Sen U.

POLISH JOURNAL OF VETERINARY SCIENCES, cilt.23, sa.2, ss.291-299, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.24425/pjvs.2020.133644
  • Dergi Adı: POLISH JOURNAL OF VETERINARY SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.291-299
  • Anahtar Kelimeler: superovulation, season, laparoscopy, plasma hormones, eCG, FSH, EQUINE CHORIONIC-GONADOTROPIN, FOLLICLE-STIMULATING-HORMONE, ESTRUS SYNCHRONIZATION, REPRODUCTIVE-PERFORMANCE, LUTEINIZING-HORMONE, PROGESTAGEN, SHEEP, PROGESTERONE, FERTILITY, INDUCTION
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Conventional methods for determining the reproductive performance of sheep bred either after estrus synchronization during the breeding season or after induction of estrus/ovulation during the non-breeding season take a long time and may give misleading results due to the effect of environmental factors. Laparoscopic observations allow real-time monitoring of ovarian activity around estrus or ovulation. This study was aimed at assessing the superovulatory effects of follicle-stimulating hormone (FSH) and equine chorionic gonadotropin (eCG) treatments by laparoscopy during breeding (September-November, n=12) and non-breeding (April-June, n=12) seasons in Akkaraman sheep. In both seasons, after CIDR withdrawal, the ewes were injected either with 600 IU eCG or 300 mu l (20 mg/ml) FSH twice at 12 hour intervals. Plasma P-4, E-2 and LH concentrations were determined at the time of intra-vaginal CIDR insertion (day 0) and then at its withdrawal (day 12), followed by 3 and 6 days of eCG or FSH injections. After 3 (first observation) and 6 (second observation) days of hormone injections, laparoscopy was performed to record ovarian activity in both seasons. The eCG increased (p<0.05) the numbers of large follicles (first observation) and CL (first and second observations) in the breeding season compared to FSH treatment. CL, small-moderate and large follicle numbers of eCG treated ewes were higher (p<0.05) than those of FSH at both observations in the non-breeding season. In the breeding season, eCG treated ewes had higher (p<0.05) plasma P-4 (3 and 6 days after hormones injections) and E-2 (3 days after hormones injections) concentrations than those of FSH. In conclusion, the results of the present study indicate that treatment with eCG during the non-breeding season can support ovarian activity, and thus increase ovulation rate and plasma hormone concentrations around induced estrus/ovulation in Akkaraman ewes.