Molecular identification and phylogeny of bovine herpesvirus-1 (BoHV-1) from cattle associated with respiratory disorders and death in Turkey

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Albayrak H., Tamer C., Özan E., Müftüoğlu B., Kadi H., Dogan F., ...Daha Fazla

MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE, cilt.76, sa.6, ss.358-361, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 76 Sayı: 6
  • Basım Tarihi: 2020
  • Doi Numarası: 10.21521/mw.6393
  • Dergi Adı: MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.358-361
  • Anahtar Kelimeler: Bovine herpes virus 1, isolate, PCR, sequencing, Turkey, VIRUS, EPIDEMIOLOGY, REGION, TYPE-1, SEMEN, BHV-1, GENE
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the presence of bovine herpesvirus 1 by molecular techniques in two cases of respiratory disease in beef cattle, reported from Amasya Province of Turkey in 2018. Nasal swab and lung tissue samples were taken. The presence of bovine herpesvirus-1 (BoHV-1) was confirmed by the PCR method using glycoprotein B (gB) gene-specific primers, and then the isolates were also subjected to partial sequencing. The results of the phylogenetic analysis revealed that the two new isolates in Turkey belonged to the same subclade as subtype 1.1 of BoHV-1, and both also had a 100% nucleotide (nt) homology with the Cooper reference strain of BoHV-1. These findings can enrich the gB sequence content data for BoHV-1 found in GenBank regarding Turkey.