Determination of Enterotoxigenic and Methicillin Resistant Staphylococcus aureus in Ice Cream

Gücükoğlu A., ÇADIRCI Ö., Terzi Gülel G., Kevenk T. O., Alişarlı M.

JOURNAL OF FOOD SCIENCE, cilt.78, sa.5, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 78 Sayı: 5
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1111/1750-3841.12093
  • Dergi Adı: JOURNAL OF FOOD SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: ice cream, S. aureus, Enterotoxigenic gene, PCR, MRSA, ANTIMICROBIAL RESISTANCE, MICROBIOLOGICAL QUALITY, EXFOLIATIVE TOXINS, MULTIPLEX PCR, CHEESE, GENES, IDENTIFICATION, PRODUCTS, MILK, MEAT
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The aim of this study was to determine the prevalence of enterotoxigenic and methicillin-resistant Staphylococcus aureus in ice creams. After culture-based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture-based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR-confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA (R)). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.