A novel biomarker explaining the role of oxidative stress in exercise and L-tyrosine supplementation: thiol/disulphide homeostasis

Kayacan Y., YAZAR H., Kisa E. C., Ghojebeigloo B. E.

ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY, cilt.124, sa.3, ss.232-236, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 124 Sayı: 3
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1080/13813455.2017.1388410
  • Dergi Adı: ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.232-236
  • Anahtar Kelimeler: Exercise, L-tyrosine, thiol, disulphide, oxidative stress, SKELETAL-MUSCLE, DAMAGE, ANTIOXIDANT, MECHANISMS, MARKER, PROTEINS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

The present study aimed to evaluate the relationship between exercise and both l-tyrosine and oxidative stress using thiol/disulphide homeostasis via a novel biomarker in rats. Following the completion of the exercise and l-tyrosine protocol, serum total thiol, native thiol, and disulphide concentrations were determined using a novel automated measurement method. Compared with the control group, serum dynamic disulphide levels were significantly lower in the E group (116.75 +/- 10.49; p <.05) and the highest in the LT group (151.0 +/- 5.84). The lowest oxidised thiol (49.75 +/- 6.18; p = .087) and the highest reduced thiol (75.38 +/- 3.16; p = .079) rates were determined to be in the E group. The highest oxidised thiol value was observed in the LT group. Exercise positively affects thiol/disulphide homeostasis, which is a novel indicator of oxidant-antioxidant parameters. Additionally, L-tyrosine appears to be more convenient combined with exercise. The new method used in our study proposes a promising, practical, and useful method for assessing the oxidative stress parameters.