Molecular characterizations of Vibrio parahaemolyticus in seafood from the Black Sea, Turkey

Gulel G., Martinez-Urtaza J.

LETTERS IN APPLIED MICROBIOLOGY, cilt.62, sa.6, ss.494-500, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 6
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1111/lam.12579
  • Dergi Adı: LETTERS IN APPLIED MICROBIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.494-500
  • Anahtar Kelimeler: Black Sea, PFGE, Real-time PCR, seafood, tdh and trh genes, V. parahaemolyticus, DIRECT HEMOLYSIN GENE, REAL-TIME PCR, COASTAL WATERS, STRAINS, TRH, PREVALENCE, DIVERSITY, GALICIA, FISH, TDH
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Vibrio parahaemolyticus is a marine bacterium that is considered as one of the major causes of bacterial food-borne outbreaks at a global scale. A total of 114 samples including mussel (n = 42), seawater (n = 22) and fish (n = 50) samples were collected and subjected to investigation. Vibrio parahaemolyticus was detected in 45 (39%) of 114 samples with an occurrence in mussel, seawater and fish samples of 76, 40.9 and 8% respectively. A total of 96 isolates were positive for the species-specific genes toxR and tlh and confirmed as V. parahaemolyticus. Presence of the virulence marker gene tdh was not identified in any of the strains investigated; however, four of strains were positive for the trh gene. Serological analysis of eight randomly selected trh-negative isolates identified three different serotypes: O4:K untypeable (KUT), O2:KUT, O3:KUT. Conversely, all four trh-positive strains belonged to a single serotype (O1:K1) and share an undistinguishable genetic profile by PFGE analysis, suggesting the existence of a dominant clone for the trh-positive strains in the region.