One-step pre-column derivatization method for HPLC-UV determination of organic acids in fruit juices

Seddiq N., Asan A.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.46, ss.192-199, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 46
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/10826076.2023.2227887
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.192-199
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

A simple and effective one-step pre-column derivatization method for reversed-phase HPLC-UV determination of the major organic acids (citric, malic, tartaric, and succinic acids) in fruit juices has been developed. The method is based on the coupling reaction of organic acids with 1-naphthylamine in the presence of N,N'-diisopropylcarbodiimide. The resulting organic acid amide derivatives were successfully separated on an AQ-C18 column and detected by a UV detector at 222 nm. Isocratic elution was performed using an acetonitrile-methanol-tetrahydrofuran-water (37:4:1:58; v/v) mixture as a mobile phase at a flow rate of 2.0 mL/min. Validation studies showed good recoveries ranging from 88.97 to 92.52% with RSD % between 2.95 and 4.56%. The detection limits obtained were 0.18, 0.48, 0.14, and 0.69 mg/mL for tartaric acid, malic acid, succinic acid, and citric acid, respectively. The method was successfully applied to three different commercial fruit juice and nectar samples with little or no clean-up procedures. [GRAPHICS] .