Akademik Veri Yönetim Sistemi
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH, cilt.6, sa.1, ss.197-202, 2012 (SCI-Expanded)
Montelukast (MLS) is used for the treatment of various allergic reactions. Viral infections are important, especially in exacerbation of allergic diseases. In this study, the apoptotic/necrotic responses of Herpes Simplex Virus (HSV-1 and Human Adenovirus (HAdV) isolate in HEp-2 and Vero cell cultures were compared with the presence or absence of non-toxic doses of pure MLS. The non-toxic dose of pure MLS for both cell lines was found to be 9.76 mu g/ml. The apoptotic cell (APC) ratios of HEp-2 cells infected with HSV and HAdV were 12.5 and 9.1% before MLS and 10.3 and 7.7% after MLS, respectively. The necrotic cell (NEC) ratio of HEp-2 cells infected with HSV and HAdV were found 26 and 20% before MLS and were found to be 22.0 and 18.7% respectively after MLS. APC ratio of Vero cells infected with HSV and HAdV were found 16 and 12.85% before MLS and were found to be 14.6 and 10.4% respectively after MLS. NEC ratio of Vero cells infected with HSV and HAdV were found to be 34 and 28.2% before MLS and were found to be 30 and 26.4% respectively after MLS. The tissue culture infectious doses (TCID50) values of HSV-1 for HEp-2 cells and Vero cells without MLS were 10-(5,25)/0.1 ml and 10-(4,95)/0.1 ml, with MLS were 10-(4,15)/0.1 and 10-(3,75)/0.1 ml, respectively. The TCID50 values of HAdV for HEp-2 cells and Vero cells without MLS were 10 -(7,0)/0.1 and 10-(6,75)/0.1 ml, with MLS were 10-(6, 25)/0.1 and 10-(5, 75)/0.1 ml, respectively. Although the methods applied in this study provides opportunities for morphologic diagnosis; results suggest the possible antiviral effect of montelukast.