Determination of Listeria monocytogenes and Serotypes in Modified Atmosphere Packed Ground and Cubed Beef Samples


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ÖZKİRAZ A., GÜCÜKOĞLU A.

Türk Tarım - Gıda Bilim ve Teknoloji dergisi, cilt.6, sa.3, ss.365-371, 2018 (Hakemli Dergi) identifier

Özet

This study was conducted to determine the Listeria monocytogenes’s presence, serotypesand resistance against various antibiotics in modified atmosphere packaged (MAP)ground and cubed beef samples. Five of ground (5/50-10%) and 3 of cubed beef samples(3/50-6%) were identified as L. monocytogenes positive in MAP samples. Eleven L.monocytogenes isolates that obtained from samples being investigated in term of hlyAgene by PCR method have verified that this gene (100%). In serotyping results, 3 of 8isolate that obtained from MAP ground beef samples were 1/2a, the other 3 isolate was1/2b and the remaining 2 isolate was 4b. Also, 1 of 3 isolate that obtained from MAPcubed beef samples were 1/2b, the other one isolate was 1/2c and the last one was 4b.One isolate against (9%) ampicillin, 2 isolate against (18.2%) chloramphenicol, 3 isolateagainst (27.2%) erythromycin, 4 isolate against (36.3%) oxytetracycline and 4 isolateagainst (36.3%) penicillin G, 6 isolate against (54.5%) tetracycline and 3 isolate against(27.2%) vancomycin was resistant in 11 L. monocytogenes isolates that confirmed byPCR. The L. monocytogenes isolates were found to be resistant to one or more antibioticsin antibiotic-resistance test results. In conclusion, applying of national residue monitoringprogram by official authority for prevention of intensive antibiotic use in order to preventthe development of resistant strains to antibiotics has great importance.