A histopathological and stereological study of the effects of acetylsalicylic acid on doxorubicin-induced hepatotoxicity in mice

Gokce A. B., Eren B., SAĞIR D., Yilmaz B. D.

BIOTECHNIC & HISTOCHEMISTRY, cilt.96, sa.4, ss.251-256, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 96 Sayı: 4
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/10520295.2020.1788724
  • Dergi Adı: BIOTECHNIC & HISTOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.251-256
  • Anahtar Kelimeler: Acetylsalicylic acid, doxorubicin, histopathology, liver, mice, stereology, VENOUS THROMBOEMBOLISM, LIPID-PEROXIDATION, VITAMIN-E, ASPIRIN, ANTHRACYCLINES, MITOXANTRONE, EFFICIENCY, APOPTOSIS
  • Ondokuz Mayıs Üniversitesi Adresli: Evet

Özet

Doxorubicin (Dox) is an anthracycline antibiotic with antineoplastic activity. Acetylsalicylic acid (Asa) is recommended for use as a prophylactic for thromboembolism during treatment of cancers. We investigated liver toxicity due to combined use of Dox and Asa in chemotherapy regimens. We used 140 Swiss albino mice divided into four main groups: control, Dox, Asa, and Dox + Asa. Each group was subdivided into seven subgroups based on time of sacrifice, i.e., 6, 12, 24, 48 h and 7, 14, 21 days. Quantitative and histopathological changes in liver were assessed by light microscopy and stereology. The portal triad area of the Dox and Dox + Asa groups was increased significantly compared to controls at 6 h, whereas in the Asa group, the means were similar to controls. Assessment of histopathology indicated an increased time-dependent degeneration and necrosis of liver tissues in mice in the Dox and Dox + Asa groups. The protective effects of Asa were not evident in Dox + Asa group. When Dox and Asa were administered together, degenerative changes were greater than for in the group that was given Dox alone. We found that Asa and Dox combined therapy increased tissue damage.